We supply a wide range of brand new equipment for the laboratory ph, petroleum product testers, freezers, furnaces, microscopes and more. Gako 2100 gako technologies unguator 2100 unguator 2100 used. Polytron pt 2100 the standard benchtop homogenizer. The mixtures were homogenized at 20,000 rpm at room temperature for 1 min with a pt 2100 polytron homogenizer kinematica ag, littauluzern, switzerland. Aug 06, 2014 kinematica polytron model pt 2100 is a bench top homogenizer and mixer and samples up to 2 liters. Samples were then dispersed by a polytron pt 2100 homogenizer kinematica ag, germany for 3 min, and ultrasonicated by means of a qsonica q700 sonicator 20 khz, qsonica llc. The kinematica polytron homogenizer model pt 2100 is a benchtop lab homogenizer for a wide range of application including dispersing, homogenizing, emulsifying, suspending, wet milling, and mixing. Samples were then dispersed with a polytron pt 2100 homogenizer kinematica ag, germany for 3 min then ultrasonicated with a qsonica q700 sonicator 20 khz, qsonica llc, newtown, ct at.
Kinematica, littau, switzerland in lysis buffer supplemented with protease and phosphatase inhibitors complete. Total rna was extracted from frozen muscle using trizol reagent. Polytron rotorstator homogenizers successfully process emulsions, suspensions and. Basal bioenergetic abnormalities in skeletal muscle from. Using the provided control software with the connection cable to pc the unit can be. Functional characteristics of gum from acacia nubica. Skip to navigation skip to content search, browse, and buy used lab equipment from biosurplus inc. For the latter, tissues were homogenized using a polytron tissue homogenizer pt 2100 in phosphatebuffered saline pbs plus protease inhibitors.
Oct, 2017 the tissue samples, homogenized with the aid of a polytron homogenizer pt 2100, kinematica polytron, newark, nj, usa were subjected to extraction of total rna using refrigerated centrifuge mikro 220r hettich zentrifugen, germany and specific kit rnaqueous 4pcr dnafreetm rna isolation for rtpcr. Polytron pt 1035 gt laboratory homogenizer accessories kinematica minimizing maintenance costs wide selection of generators enables homogenizations from 0. The tissue was pulverized to powder under liquid nitrogen and homogenized in ripa buffer 50 mm trishcl, ph 7. Category homogenizers 20 molecular biology equipment 4. Enzymatic incubation was performed at 50 c under mild agitation for 16 h. This manual describes operation of the kinematica polytron pt 2100. Due to the nature of used and surplus equipment no guarantees or warranties either expressed or implied are offered unless specifically noted in the description.
Total rna was extracted by means of a trizol gibco brl reagent, using a polytron homogenizer polytron pt 2100, kinematica ag, and purified with phenol. Polytron pt 2500 e the new standard homogeniser labmate online. Kinematica polytron pt 2100 homogenizers, model 276516. Pdf effect of date palm pollen on serum testosterone and. Find the best prices on used kinematica instruments, equipment, and accessories. Thanks to their small diameter, polytron 2 polytron drive unit ptmr 2100 aggregates can even reach to bottom of small test tubes.
At the termination of the study, tumors from three representative mice in each group were harvested and homogenized using a polytron system pt 2100 homogenizer brinkmann instruments, mississauga, ontario, canada in ripa lysis buffer, minus detergents. The pt 0 d counts within the polytron handheld range of dispersing systems for the laboratory 0. Sonicated samples were vortexed and extracted using 200. Drive and control unit the polytron pt 0 d handheld homogenizer is equipped with a 100 watt powered drive system and control unit which is supplied with 24v safety current by the power and supply unit. Samples were then dispersed using a polytron pt 2100 homogenizer kinematica ag, luzern, switzerland for 3 min, and ultrasonicated by means of a qsonica q700 sonicator 20 khz, qsonica llc. Kinematica polytron pt2100 benchtop homogenizer rotor speed. Kinematica polytron pt 2100 homogenizers, model 276516, eachhomogenizers are used to rapidly break down plant and animal tissue for chemical analysis, prepare emulsions and samples for titrations, and extract pesticides in soil samples. Predictive biomarkers for response to exercise medical. Feb 04, 2010 total rna was extracted from frozen muscle using trizol reagent. Kinematica, littau, switzerland in lysis buffer consisting of 20 mm tris, 7 mm nacl. The results of the colour profile analysis were tabulated using easymatch software. Rheological and structural properties of hemiramphus far. Kinematica polytron easyclean dispersing aggregates for pt.
Translational regulation of glur2 mrnas in rat hippocampus. Kinematica polytron easyclean dispersing aggregates for pt 2100 homogenizers 7mm generator. Effect of date palm pollen on serum testosterone and intratesticular environment in male albino rats. Kinematica polytron pt 2100 homogenizers, model 276516, each. Influence of the carbohydratebinding module on the activity. Kinematica, littau, switzerland in lysis buffer consisting of 20 mm tris, 7 mm nacl, 10% volvol glycerol, 1% volvol nonidet p40, and 2 mm ethylenediaminetetraacetic acid and supplemented with protease and phosphatase inhibitors complete. The reference sample was submitted to the same treatment but did not contain. Mammarydigitalnail syndrome is a novel phenotypic association consisting of anonychia onychodystrophy with hypoplasia or absence of distal phalanges in males and females, accompanied by juvenile. Gestation agerelated increase in 50kda rat uterine. Kinematica polytron pt mr 2100 homogenizer with stand for. Bioengineering free fulltext topical digitoxigenin. The comparative efficacy of reninangiotensin system blockers.
Briefly, a fish sample 5 g was dispersed in 5% trichloroacetic acid solution 20 ml and homogenized in a polytron homogenizer pt 2100, kinematica ag, switzerland for 5 min. Shop online for a wide selection of kinematica polytron easyclean dispersing aggregates for pt 2100 homogenizers equipped with sawteeth, which. Effects of aerobic training on gene expression in skeletal. Emulsion was prepared using a polytron pt2100 homogenizer at 26. Left ventricular diastolic dysfunction and myocardial.
A unique non corrosive quick coupling allows the changing of dispersing aggregates even with one hand. Homogenizer and provides information on installation, maintenance, parts and accessories. Shop online for a wide selection of kinematica polytron homogenizer stands plate stand for safe and easy height adjustment of the drive unit. Shop online for a wide selection of kinematica polytron model pt 1035 gt pt 3100 d homogenizer accessory, generator powerful and versatile benchtop homogenizer. A transcriptional map of the impact of endurance exercise.
Influence of the carbohydratebinding module on the. Remove control knob 12 with crossslotted screw driver. Brinkmann pt1035 polytron homogenizer hitechtrader used. Manufacturing automation and intelligence engineers polytron. The organ contents were collected by flushing of the cecum with 10 ml sterile 1 x pbs. The yeast geotrichum candidum encodes functional lytic. Download scientific diagram vortex, syringeextrusion and homogenizer. Kinematica polytron model pt2100 is a bench top homogenizer and mixer and samples up to 2 liters. Lv samples were homogenized with a polytron homogenizer pt 2100.
Investigation of the tissue distribution and physiological. Effect of spraydrying with organic solvents on the. Kinematica polytron model pt 1035 gt pt 3100 d homogenizer. An in vivo evaluation of docetaxel delivered intratumorally. Mammarydigitalnail syndrome is a novel phenotypic association consisting of anonychia onychodystrophy with hypoplasia or absence of distal phalanges in. Vortex, syringeextrusion and homogenizer equipment used in this. The polytron pt 2500 e extends our standard line small for the small sized sample preparation in the lab lab small, 0. It is ideal for the rapd break down of plant and animal tissue for chemical analysis, for the preparation of emulsions and the. No matter which company i work for or the manufacturing, packaging or process solution i need, polytron is my first choice and preferred partner to help me and my employer be successful. The benefit of intraperitoneal chemotherapy for the. A polytron pt 2100 homogenizer glenmills, clifton, nj fitted with a polytron ptda 21122ec probe was used.
The muscle was homogenized in a polytron homogenizer pt 2100 using a microattachment at 11,000 rpm using one to three 5s bursts. Samples were then dispersed with a polytron pt 2100 homogenizer kinematica ag, germany for 3 min then ultrasonicated with a qsonica q700 sonicator 20 khz, qsonica llc, newtown, ct at 350 w ultrasound power for 3 min. Biosurplus has the most comprehensive and available list of used laboratory equipment online. Kinematica polytron pt 2100 homogenizer rent, finance, or buy. Kinematica pt3100 polytron pt 3100 polytron pt3100 homogenizer polytron.
Kinematica, lucern, switzerland at 11,000 rpm for 1 min. Proxio group global online only auction surplus lab. The method according to the present invention can be implemented using software that is commercially available to measure gene expression in connection with a microarray. The mixed solution was vortexed vigorously for 1 minute.
Polytron pt 2100 units are designed to homogenise, emulsify, blend and mix small to medium quantities of organic and inorganic materials. Search, browse, and buy used lab equipment from biosurplus. Polytron pt 2100 homogenizers, kinematica homogenizers benchtop homogenizers homogenizers are used to rapidly break down plant and animal tissue for chemical analysis, prepare emulsions and samples for titrations, and extract pesticides in soil samples. Samples of 300 mg wet lung tissue were homogenized with a polytron homogenizer polytron pt2100 homogenizer, kinematica ag, luzern, switzerland for 60 s in 1 ml of sodium phosphate buffer, ph 6. Loss of cnfy toxininduced inflammation drives yersinia. Polytron homogenizer pt 3100 with stand polytron pt3100. Preparation of fish oil microparticles by conventional spray drying. Lung tissue was homogenized in lysis buffer 50 mm n2hydroxyethylpiperazinen. Frozen pieces were homogenized for 60 s in 1 ml of trizol using a 7mm polytron aggregate ptda 2107, kinematica ag, switzerland adapted to a polytron homogenizer pt2100 running at maximum speed. Kinematica polytron benchtop homogenizers combine quality, convenience and high performance for rapid, reproducible sample processing and consistent sampletosample uniformity. Recombinant human insulinlike growth factor binding. Relationships between early inflammatory response to. For molecular studies, the homogenate was diluted with an equal volume of 2. Switzerland adapted to a polytron homogenizer pt2100 running at maximum speed.
Frozen pieces were homogenized for 60 s in 1 ml of trizol using a 7mm polytron aggregate model ptda 2107, kinematica adapted to a polytron homogenizer model pt2100 running at maximum speed. Lipid material, which packed at the surface of the supernatant, was removed and the. Lysates were heatdenaturated for 4 min and loaded on 412% nupage gels invitrogen. The homogenate was centrifuged, and the acetonitrile extract was removed and evaporated as with the plasma samples. This model replaces the pt 1600 e and the pt 2100 incorporating the best aspects of both models. Polytron dispersing aggregates for the pt 6100 d have shafts. Kinematica polytron pt mr 2100 homogenizer with standexcellent used condition. Aliskiren effect on nonalcoholic steatohepatitis in. These generators work via the rotorstator system, in which samples are drawn into the center of the generator tip, vigorously mixed, accelerated, pressed through the. The ceca were weighed and homogenized in pbs at 22. Frequency, suppressive capacity, recruitment and induction. Polytron pt 1035 gt laboratory homogenizer generators kinematica minimizing maintenance costs wide selection of generators enables homogenizations from 0.
Using molecular classification to predict gains in maximal. Tissues were either fixed in 15% vv neutralbuffered formalin for immunohistochemistry or snapfrozen and stored at. Dispersion and homogenization of pumpable fluid products in accordance with the. Polytron s project management methods are the best in the market. Software 0 system 0 video conferencing system 0 electrophoresis 27 accessory 1 autorad cassette 0 controller 0. System polytron pt 1035 gt system polytron pt 3100 d. The generators are also designated quickconnect as they can be connecteddisconnected from the homogenizer quickly and easily. This is a preoowned kinematica benchtop homogenizer polytron dispering system pt 2100 in excellent condition. Shop page 2 of 4 innovative trend lab equipment instrument. If you want to use the equipment while the actual work from 17. Gestation agerelated increase in 50kda rat uterine calcium. Peritoneum samples were preweighed and homogenized in 1 ml 75% 0.
Tissue samples were homogenized with the aid of a polytron homogenizer pt 2100, kinematica polytron, newark, nj, usa and subjected to extraction of total rna using refrigerated centrifuge mikro 220r hettich zentrifugen, germany and specific kit rnaqueous 4pcr dnafree tm rna isolation for rtpcr. Large cell debris and nuclei were pelleted by centrifuging at 600. Polytron 11control kinematica pcu stirrer pt1035 homogenizer 66. Pdf activation of the neuregulinerbb system during. Frozen pieces were homogenized for 60 s in 1 ml of trizol using a 7mm polytron aggregate model pt da 2107, kinematica adapted to a polytron homogenizer model pt 2100 running at maximum speed. Total rna was extracted using the rneasy lipid tissue mini kit qiagen gmbh. Stand model, volume range 0,05 to 2500 ml for dispersing, homogenising, extractions, cell disruption, mixing, emulsifying and suspending. Kinematica 11010025 pt 1200e motor model polytron handheld homogenizer, 0. Frozen pieces were homogenized for 60 s in 1 ml of trizol using a 7mm polytron aggregate pt da 2107, kinematica ag, switzerland adapted to a polytron homogenizer pt 2100 running at maximum speed. These homogenizers include support stand for the bench top models. Measurements were carried out at room temperature and samples were. Apr 03, 2014 two samples were available for each subject, one taken preexercise and a second one taken postexercise.
Brinkmann manufactured by kinematica ag polytron homogenizer. Laboratory line homogenizing of cabbage, spinach and celery. May 01, 2006 the tissue was pulverized to powder under liquid nitrogen and homogenized in ripa buffer 50 mm trishcl, ph 7. Kinematica 11010025 pt 1200e motor model polytron handheld. A polytron pt 2100 homogenizer glenmills, clifton, nj fitted with a polytron pt da 21122ec probe was used. Kinematica polytron model pt mr 2100 homogenizer youtube. Polytron membantu anda menemukan jajaran produk elektronik dengan teknologi terkini termasuk audio video, home appliances, smartphone, dan lainnya. The benefit of intraperitoneal chemotherapy for the treatment. Lytic polysaccharide monooxygenases disrupt the cellulose. Frozen pieces were homogenized for 60 s in 1 ml of trizol using a 7 mm polytron aggregate pt da 2107, kinematica ag, switzerland adapted to a polytron homogenizer pt 2100 running at maximum speed. The sturdy, powerful and versatile polytron pt 2100 counts within the polytron benchtop homogenizer systems for the laboratory range small 0. For use with equipment, polytron pt 2100 homogenizer.
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